HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPCL) ANALYSIS OF CUCURBITACINS IN SOME BRYONIA SPECIES

Gülnur TOKER*°, Nurgün ERDEMOGLU*, M.Cihat TOKER**

*Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330 Etiler, Ankara, TURKEY.
**Ankara University, Faculty of Science, Department of Biology, 06100 Tandogan, Ankara, TURKEY.
°Corresponding Author

Summary:
In this study,the roots of Bryonia multiflora Boiss.&Heldr.(Cucurbitaceae)growing in Central, South and East Anatolia was analysed from the aspect of cucurbitacin B and cucurbitacin I in HPLC.The results were compared with those parts of known official B. alba species.HPLC analysis were achieved on Lichrospher 100 RP C18 e column by using an isocratic mixture of acetonitrile:water (2:3) at a flow rate of 1ml/min as a mobile phase.The UV detection was performed at 230 nm. It was found that cucurbitacin B had a high ratio in chloroform and low in methanol extracts.On the contary, cucurbitacin I had a high ratio in methanol and low in chloroform extracts.Cucurbitacin B was found to be 0.022% and trace and cucurbitacin I 0.021 % and 0.125% in the B.multiflora and B.alba roots respectively.

Key words:
Bryonia multiflora, B.alba, Cucurbitacin, HPLC.