SYNTHESIS AND STRUCTURAL CHARACTERIZATION OF SOME 5(6)-SUBSTITUTED-2-HYDROXYMETHYLBENZIMIDAZOLE DERIVATIVES AND THEIR PLATINUM (II) COMPLEXES AND DETERMINATION OF THEIR IN VITRO ANTITUMOR ACTIVITIES BY ‘REC-ASSAY’ TEST
Fatma GÜMÜS* °, Fatih IZGÜ**, Öztekin ALGÜL*
*Gazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330 Etiler, Ankara, TURKEY. **METU, Faculty of Science and Arts, Department of Biology, Ankara-TURKEY.
°Corresponding Author

Summary:
In this study, three 5(6)-non/-chloro/-methylsubtituted-2-hydroxymethylbenzimidazoles (L1-L3 respectively) and their three new plantium (II) complexes (C1-C3) were synthesized. The ligands were obtained by condensing 4-non/-chloro/-methylsubstituted -1,2- phenylenediamine with glycolic acid in the presence of 4.5 N HCl. The new two complexes of type [Pt (L1/or L2)2 Cl2] 2H2O (where L1 and L2 behaved as a monodentat ligands) and one complex of type Pt L3`Cl2 (Where L3` desprotone form of L3 behaved as a bidentat ligand) were obtained by the reaction of the ligands with K2PtCl4. The chemical structure of the Pt (II) complexes of the benzimidazole derivatives were characterized by their elemental analyses data and their IR and 1H NMR spectra comparing with those of the ligands. All the ligands and complexes were tested for their preliminary in vitro antitumor activity with ‘Rec-Assay’test. It can be said that in contrast to the ligands (L1-L3) and complex C3, C1 and C2 had promising antitumor activity. Further work is required to confirm their in vitro antitumor activities.

Keywords:
2-Hydroxymethylbenzimidazoles, Platinum (II) complexes, In vitro Antitumor Activity, Rec-Assay Test