QUALITATIVE AND QANTITATIVE STUDIES ON THE DRUGS CONTAINING N-SUBSTITUTED TRIAZOLE STRUCTURES

Fatmagül SELÇUK, Okan ATAY*,o, Ningur NOYANALPAN*

*Gazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330 Etiler, Ankara, TURKEY.
oCorresponding Author

Summary:
In this publication,qualitative and quantitative studies have been performed on a variety of commercial preparations that contain fluconazole and itraconazole that are essentially N-substituted triazole derivatives which are used in systemic mycosis in recent years.
Fluconazole and itraconazole have been analysed qualitatively along with other antifungal imidazole derivatives using thin layer chromatography(TLC),densitometric and high pressure liquid chromatography(HPLC). In TLC,20 different solvent systems have been tried and suitable Rf values have been determined for active ingredients.In these studies,differentiating reagents have been used for active ingredients.
In studies made by using HPLC method, fluconazole,itraconazole,clotrimazole,myconazole nitrate,oxiconazole nitrate,ketoconazole and tioconazole have been analysed under the working conditions developed by us.Only the retention times of tioconazole and itraconazole have been found to be very close to each other.
In quantitative studies, ultraviolet spectroscopy ,HPLC and densitometric methods have been used.In UV spectroscopy method,the absorbance value at 261 nm in methanol has been used for both of the active compounds. Quantitative determinations have been performed by using the regression equations calculated before. In quantitative determinations done with HPLC, ketoconazole has been used as the internal Standard.
Silicagel 60 GF254 coated plates have been applied in densitometric studies and the regression equations calculated by using the peak areas of the active compounds applied at varying concentrations, measured at 261 nm, have been used for quantitative calculations.
The results obtained with three different methods have been compared using(Student t and Fisher f test)

Key words:
N-Substituted Triazole, Imidazole, Fluconazole, Itraconazole, TLC, Densitometry, HPLC, UV Spectroscopy, Qualitative and quantitative determination.